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Buffers

Buffers

Buffers maintain their pH when small amounts of strong acids or bases are added. In biology, buffer solutions are used to provide a constant pH environment for biochemical processes. The pH often changes the environments of tissues and cells and can alter the activity of biomolecules. Buffers maintain the correct pH for proteins and oligonucleotides, and are especially important for techniques like electrophoresis.

Commonly used buffers in life science research share some of these characteristics:

  • A pKa value between 6.0 and 8.0
  • High water solubility
  • Minimal changes due to temperature and concentration
  • Limited effects due to ionic or salt composition of the solution

Browse our comprehensive selection of buffers including common buffers tris(hydroxymethyl)aminomethane (tris) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES).

Magnesium Chloride

Magnesium Chloride

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Phenol, Saturated Solution, Ph 4.5

Phenol, Saturated Solution, Ph 4.5

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Phenol : Chloroform Saturated Solution, Ph 5.2

Phenol : Chloroform Saturated Solution, Ph 5.2

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Phenol:Chloroform Saturated Solution, Ph 6.7

Phenol:Chloroform Saturated Solution, Ph 6.7

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Tris-Glycine Buffer

Tris-Glycine Buffer

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Formamide Deionized

Deionized Formamide is used in molecular biology and a denaturing agent for nucleic acids in gel electrophoresis orr hybridization experiments. In the latter case the role of formamide is to decrease the temperature necessary for the reassociation of complementary nucleic acids.

GLYCOGEN

Glycogen is an inert material and does not interfere with molecular biology experiments. It can replace tRNAs or sonicated DNAs as an additive for the precipitation of nucleic acids.

10X PBD (fr)

10X Phosphate-Buffered Detergent.

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SDS EASY SOL 20% (fr)

SDS is an anionic detergent typically used to solubilize and denature proteins for electrophoresis. Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS.It is RNAse,DNAse, Protease free with an assay value of 20%.This solution is provided as a convenient ready-to-use firmat which eliminates the need to handle powdered SDS.

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TAE 50X BUFFER--N/H

Buffer for Electrophoresis.

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Urea, Ultra-Pure (1lb Size)

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.

TBE Modifier mixture, 15 mL

Proprietary mixture of concentrated salts for removing DNA from TBE-buffered gels

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Triethanolamine Hydrochloride

Triethanolamine Hydrochloride

Tris Hydrochloride

Tris Hydrochloride

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Potassium Chloride

Potassium Chloride

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Hepes Hemisodium Salt

Hepes Hemisodium Salt

Pipes Sesquisodium Salt

Pipes Sesquisodium Salt

Manganese Sulfate Monohydrate

Manganese Sulfate Monohydrate

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CHAPSO

CHAPSO is the hydroxy analog of CHAPS; a nondenaturing zwitterionic detergent for solubilizing proteins, especially integral membrane proteins while maintaining the functional capability of the protein of interest. It has a more polar head group than CHAPS, giving it a greater solubility. CHAPSO has a high CMC (8 mM) and does not denature membrane-bound proteins. It can solubilize the opiate receptor in a state exhibiting reversible binding of opiates.

Magnesium Chloride Hexahydrate

Magnesium Chloride Hexahydrate

Magnesium Sulfate Heptahydrate

Magnesium Sulfate Heptahydrate

Potassium Chloride

Potassium Chloride

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Potassium Phosphate, Monobasic

Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic, and tribasic (K3PO4). Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.

Potassium Phosphate Dibasic

Potassium phosphate is a reagent with high buffering capacity. It occurs in several forms: monobasic, dibasic and tribasic. Most pH neutral potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms to varying degrees, depending on the desired pH.

Calcium Phosphate Dibasic

Calcium Phosphate Dibasic

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HEPES Sodium Salt--N/H

HEPES has been described as one of the best all-purpose buffers available for biological research. At most biological pHs the molecule is zwitterionic.

Tris Hydrochloride

Tris Hydrochloride

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Choline Chloride

Choline chloride is an organic compound and a quaternary ammonium salt. It is a weak acid. Choline chloride is the salt of the naturally occurring choline, the pre-stage of the neurotransmitter acetylcholine, which is important for mnemonic and thought-processes. Choline occurs naturally in fungi, hop and kingcups and as integral part of lecithin. Choline chloride is a common food additive in animal husbandry

Magnesium Chloride Hexahydrate

Magnesium Chloride Hexahydrate

Manganese Sulfate Monohydrate

Manganese Sulfate Monohydrate

Sodium Phosphate Monobasic

Sodium Phosphate Monobasic

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Guanidine Hydrochloride

Guanidine hydrochloride consists of a networkof guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds,it is a strong chaotropic agent. Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a free flowing powder with solid material dispersed throughout, or a solid. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine HCl appear identical.

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HEPES

HEPES is a zwitterionic organic chemical buffering agent.

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Hepes Sodium Salt

HEPES is a zwitterionic organic chemical buffering agent.

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Imidazole Un3263-III

Imidazole is a histamine antagonist.

Magnesium Sulfate, Heptahydrate

Magnesium Sulfate, Heptahydrate

Potassium Chloride

Potassium Chloride

Sodium Phosphate Dibasic

Sodium Phosphate Dibasic

Sodium Phosphate Monobasic

Sodium Phosphate Monobasic

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Sodium Phosphate Dibasic

Sodium Phosphate Dibasic

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Ammonium Acetate, 7.5 M Solution

Ammonium Acetate, 7.5 M Solution

Sodium Phosphate Monobasic, Monohydrate

Sodium Phosphate Monobasic, Monohydrate

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Tris Hydrochloride

Tris Hydrochloride

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Tris-Borate-Edta Buffer

Tris-Borate-Edta Buffer

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Urea, Ultra-Pure (1lb Size)

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.

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DEPC-TREATED WATER

DEPC Trested Water is designed for applications where DNase and RNase contamination are of concern. While auotclaving alone can sufficiently inactivate RNases for many applications, water that is treated with diethylpyrocarbonate (DEPC) is often prefferable as added precaution. This product is deionized water that has been DEPC treated, then filtered and autoclaved to provide a ready-to-use reagent that can be used with confidence.

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TBE 10X BUFFER (1L) (fr)

TBE buffer maintains the structural integrity of nucleic acids and more suitable for their size analysis. TBE has a greater buffering capacity and will give sharper resolution. TBE is better suited for high-voltage (>150 V) electrophoresis because of its higher buffering capacity.

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TRIS BASE

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.

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}{TWEEN 20

TWEEN 20 is a polyoxyethylene sorbitol ester.

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Bis-Tris Propane, UltraPure

Bis-Tris Propane, UltraPure

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SODIUM PHOSPHATE DIBASIC ANHY.

Sodium phosphate dibasic anhydrous is a sodium salt of phosphoric acid. It is a white powder that is highly hygroscopic and water soluble.