Rat Liver S9 Homogenate In KCL

Rat Liver S9 Homogenate In KCL

Synonyms S9 from Liver

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SKU 085041-CF
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R$136.85
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Description

Product Description

Rat Liver S9 Homogenate is a Supernatant fraction obtained from an organ (usually liver) homogenate by centrifuging at 9000 g for 20 minutes in a suitable medium; this fraction contains cytosol and microsomes. The microsomes component of the S9 fraction contain cytochrome P450 isoforms (phase I metabolism) and other enzyme activities. The cytosolic portion contains the major part of the activities of transferases (phase II metabolism).

Application Notes

Rat Liver S9 Homogenate is used in Ames Mutagen Assay, to assess the mutagenic potential of chemical compounds. It is also used in assays that measure the metabolism of drugs and other xenobiotics. Many xenobiotics, neurotransmitters, steroids, and other hormones are metabolized by sulfate conjugation, a reaction catalyzed by the class of sulfotransferase enzymes. The common sulfate group donor is adenosine 3’-phosphate 5’-phosphosulfate.

Key Applications

Immunoassays | Ames Mutagen Assay | Study of xenobiotic metabolism and drug interactions

Specifications
SKU 085041-CF
Alternate Names S9 from Liver
Application Notes Rat Liver S9 Homogenate is used in Ames Mutagen Assay, to assess the mutagenic potential of chemical compounds. It is also used in assays that measure the metabolism of drugs and other xenobiotics. Many xenobiotics, neurotransmitters, steroids, and other hormones are metabolized by sulfate conjugation, a reaction catalyzed by the class of sulfotransferase enzymes. The common sulfate group donor is adenosine 3’-phosphate 5’-phosphosulfate.
Applications Immunoassays | Ames Mutagen Assay | Study of xenobiotic metabolism and drug interactions
Physical Appearance Frozen liquid
Preparation Method Preparation: The material is prepared from 8-10 week old Sprague Dawley male rats obtained from Charles River Breeders. The animals are quarantined for two weeks prior to use. Preparation of S9 is by the procedure describes by Ames, et. Al. Induction: Animals induced with Aroclor 1254 are administered one intraperitoneal (i.p.) injection of Aroclor 1254 (500 mg/kg) suspended in corn oil five days prior to sacrifice, and starved 12 hours prior to sacrifice. Animals induced with 3-methylcholanthrene are administred three daily i.p. injections 24 hours after the last injection.
Product Families Description Rat Liver S9 Homogenate is a Supernatant fraction obtained from an organ (usually liver) homogenate by centrifuging at 9000 g for 20 minutes in a suitable medium; this fraction contains cytosol and microsomes. The microsomes component of the S9 fraction contain cytochrome P450 isoforms (phase I metabolism) and other enzyme activities. The cytosolic portion contains the major part of the activities of transferases (phase II metabolism).
Source Species: Rat; Strain: Sprague Dawley; Sex: Male; Tissue: Liver
Storage and Handling The S9 preparations are shipped in dry ice. Upon receipt; the S9 must be transferred to storage at -70°C or below.
Trade Name Cappel™