IgG2B (Kappa), Mouse, Ascites,

IgG2B (Kappa), Mouse, Ascites,

Synonyms Mouse IgG2b/k

MDL Number: MFCD00164802

Packaging size: 5 mg

SKU 0850344
Price:
R$248.85
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Description

Product Description

Product is the lyophilized powder of clarified mouse myeloma ascites IgG2b (j) MOPC 195.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. Purified proteins are recommended as ELISA coating antigens and as controls.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunoblot| Immunohistochemistry

Specifications
SKU 0850344
Alternate Names Mouse IgG2b/k
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Each ascites is tested for immunoglobulin content by radial immunodiffusion with specific antisera following precipitation by ammonium sulfate.
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. Purified proteins are recommended as ELISA coating antigens and as controls.
Clone Name MOPC 195
Conjugate Unconjugated
Formulation Details Lyophilized
Host Mouse
Isotype IgG2b, kappa
Applications Immunoassays | Immunoblot| Immunohistochemistry
MDL # MFCD00164802
Pack Size 5 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Lyophilized powder
Preparation Method The IgG2b (j) MOPC 195 tumor line is a mineral oil (Bayol F)-induced plasmacytoma. The clarified ascites is produced in BALB/c mice, and is filtered through a 0.22 µm filter, vialed and lyophilized
Presentation lyophilized
Research Areas Immunoassays
Solubility Reconstitution: Reconstitute product with 1 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Specificity The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Each ascites is tested for immunoglobulin content by radial immunodiffusion with specific antisera following precipitation by ammonium sulfate (See Protocol for Immunoglobulin Precipitation). In addition; the ascites contains the normal threshold of mouse immunoglobulins and ascites proteins.
Sterility Filtered through a .22 µm filter
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 1 week at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.